{"id":64,"date":"2017-04-13T19:29:46","date_gmt":"2017-04-13T19:29:46","guid":{"rendered":"https:\/\/scholarblogs.emory.edu\/dyerlab\/?page_id=64"},"modified":"2017-10-27T21:22:31","modified_gmt":"2017-10-27T21:22:31","slug":"spectroscopy","status":"publish","type":"page","link":"https:\/\/scholarblogs.emory.edu\/dyerlab\/capabilities\/spectroscopy\/","title":{"rendered":"Spectroscopy"},"content":{"rendered":"<h1><span style=\"font-size: 12pt\"><strong>Current Capabilities<\/strong><\/span><\/h1>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"size-medium wp-image-295 alignright\" src=\"https:\/\/scholarblogs.emory.edu\/dyerlab\/files\/2017\/04\/spec1-273x300.png\" alt=\"\" width=\"273\" height=\"300\" srcset=\"https:\/\/scholarblogs.emory.edu\/dyerlab\/files\/2017\/04\/spec1-273x300.png 273w, https:\/\/scholarblogs.emory.edu\/dyerlab\/files\/2017\/04\/spec1.png 296w\" sizes=\"auto, (max-width: 273px) 100vw, 273px\" \/><\/p>\n<ul>\n<li style=\"text-align: left\"><span style=\"font-size: 12pt\">Time-resolved IR (SS-FTIR; QCL based TRIR; ultrafast IR)<\/span><\/li>\n<li style=\"text-align: left\"><span style=\"font-size: 12pt\">TR Raman spectroscopy &amp; imaging<\/span><\/li>\n<li style=\"text-align: left\"><span style=\"font-size: 12pt\">FTIR imaging with MCT FPA<\/span><\/li>\n<li style=\"text-align: left\"><span style=\"font-size: 12pt\">TR fluorescence, FCS &amp; imaging<\/span><\/li>\n<\/ul>\n<p><span style=\"font-size: 12pt\"><strong>New Methods Development&nbsp;<\/strong><\/span><\/p>\n<ul>\n<li><span style=\"font-size: 12pt\">Microfluidics Ultrafast Mixing<\/span><\/li>\n<li><span style=\"font-size: 12pt\">Isotope Edited IR<\/span><\/li>\n<li><span style=\"font-size: 12pt\">Photo-triggering using caged reagents<\/span><\/li>\n<li><span style=\"font-size: 12pt\">Laser-induced pH jump<\/span><\/li>\n<li>Laser-induced potential jump<\/li>\n<\/ul>\n<div id=\"info\">\n<p><span style=\"font-size: 12pt\">Measuring fast reaction kinetics: microfluidic flow-mixing, hyperspectral FTIR measurements of the&nbsp;<\/span><span style=\"font-size: 12pt\">l<\/span><span style=\"font-size: 12pt\">ight induced turnover of photosystem II (PSII) are sho<\/span><span style=\"font-size: 12pt\">wn to&nbsp;<\/span><span style=\"font-size: 12pt\">the right. <\/span><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"size-medium wp-image-296 alignright\" src=\"https:\/\/scholarblogs.emory.edu\/dyerlab\/files\/2017\/04\/spec2.png\" alt=\"\" width=\"250\" height=\"154\"><span style=\"font-size: 12pt\">The visible &nbsp;<\/span><span style=\"font-size: 12pt\">image (panel A) shows the IR microfl<\/span><span style=\"font-size: 12pt\">uidics cell (channels ~ 50 \u00b5m), with the sample stream flowing from right to left. The sheath buffer streams also enter from the right in a &#8220;Y&#8221; configuration. The P<\/span><span style=\"font-size: 12pt\">SII sample is visible as a green-colored stream that is focused to a jet by the sheath buffer flow. The central square represents the region imaged by the FTIR m<\/span><span style=\"font-size: 12pt\">icroscope. Panel B shows the IR image plotted at the maximu<\/span><span style=\"font-size: 12pt\">m of the amide I absorbance at 1655 cm<sup>-1<\/sup>, where red corresponds to maximum absorbance (~100 mOD) and blue corresponds to minimum absorbance. The FTIR image shows the distribution of the protein in the microfluidics flow, illustrating the hydrodynamic focusing of the sample stream. The FTIR spectrum at a single pixel along the jet is shown in panel C. This spectrum shows the protein amide I and II modes and the very low noise level of the measurement (~1mOD). As the PSII sample flows into the lighted observation region, it begins to turn over and the FTIR spectrum evolves as the flow moves down the microfluidics channel. Thus, the FTIR spectra along the flowing stream represent the time-evolution of light-induced O2 production by PSII, with 5 \u00b5s time resolution.<\/span><\/p>\n<\/div>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Current Capabilities Time-resolved IR (SS-FTIR; QCL based TRIR; ultrafast IR) TR Raman spectroscopy &amp; imaging FTIR imaging with MCT FPA TR fluorescence, FCS &amp; imaging New Methods Development&nbsp; Microfluidics Ultrafast Mixing Isotope Edited IR Photo-triggering using caged reagents Laser-induced pH jump Laser-induced potential jump Measuring fast reaction kinetics: microfluidic flow-mixing, hyperspectral FTIR measurements of the&nbsp;light &hellip; <\/p>\n<p class=\"link-more\"><a href=\"https:\/\/scholarblogs.emory.edu\/dyerlab\/capabilities\/spectroscopy\/\" class=\"more-link\">Continue reading<span class=\"screen-reader-text\"> &#8220;Spectroscopy&#8221;<\/span><\/a><\/p>\n","protected":false},"author":4543,"featured_media":0,"parent":29,"menu_order":3,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"ngg_post_thumbnail":0,"footnotes":""},"class_list":["post-64","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/scholarblogs.emory.edu\/dyerlab\/wp-json\/wp\/v2\/pages\/64","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/scholarblogs.emory.edu\/dyerlab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/scholarblogs.emory.edu\/dyerlab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/scholarblogs.emory.edu\/dyerlab\/wp-json\/wp\/v2\/users\/4543"}],"replies":[{"embeddable":true,"href":"https:\/\/scholarblogs.emory.edu\/dyerlab\/wp-json\/wp\/v2\/comments?post=64"}],"version-history":[{"count":19,"href":"https:\/\/scholarblogs.emory.edu\/dyerlab\/wp-json\/wp\/v2\/pages\/64\/revisions"}],"predecessor-version":[{"id":589,"href":"https:\/\/scholarblogs.emory.edu\/dyerlab\/wp-json\/wp\/v2\/pages\/64\/revisions\/589"}],"up":[{"embeddable":true,"href":"https:\/\/scholarblogs.emory.edu\/dyerlab\/wp-json\/wp\/v2\/pages\/29"}],"wp:attachment":[{"href":"https:\/\/scholarblogs.emory.edu\/dyerlab\/wp-json\/wp\/v2\/media?parent=64"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}