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Rhizosphere Inspired Natural Products

The rhizosphere microbiome is a diverse microbiome that is sustained by an equilibrium of both mutualistic and parasitic relationships of its inhabitant microorganisms. This coexistence entices the study of small molecules produced in such environments and how they facilitate survival. Our interest lies in promysalin, a small molecule isolated from the rhizosphere in 2011. Promysalin is a potent species-specific inhibitor of Gram-negative pathogen, Pseudomonas aeruginosa. Our work began with determining the natural stereochemistry via synthesis and biological assays which lead to a DTS guided analog library, and has recently culminated in target identification.

Keohane, C.E.; Steele, A.D.; Fetzer, C.; Khowsathit, J.; Van Tyne, D.; Moynie, L.; Gilmore, M.S.; Karanicolas, J.; Sieber, S.A.; Wuest, W.M.* “Promysalin Elicits Species-Selective Inhibition of Pseudomonas aeruginosa by Targeting Succinate Dehydrogenase” J. Am. Chem. Soc. 2018 ASAP DOI10.1021/jacs.7b11212

Steele, A.D.; Keohane, C.E.; Knouse, K.W.; Rossiter, S.E.; Williams, S.J.; Wuest, W.M.* “Diverted Total Synthesis of Promysalin Analogs Demonstrates that an Iron-Binding Motif is Responsible for its Narrow-spectrum Antibacterial Activity” J. Am. Chem. Soc. 2016 138, 5833.  DOI: 10.1021/jacs.6b03373

Keohane, C.E.; Steele, A.D.; Wuest, W.M.* “The rhizosphere microbiome: A playground for natural product chemists” Synlett 2015 26, 2739. DOI: 10.1055/s-0035-1560711 (invited Synpact Paper)

Steele, A.D.; Knouse, K.W.; Keohane, C.E.; Wuest, W.M.* “Total Synthesis and Biological Evaluation of (-)-Promysalin” J. Am. Chem. Soc. 2015 137, 7314DOI: 10.1021/jacs.5b04767