Ongoing research in the Goldberg lab seeks to define mechanisms of tolerance, coexistence, and competition by investigating lab strains and clinical isolates of Staphylococcus aureus and Pseudomonas aeruginosa through in vitro experimental evolution, genomic analysis, and we are currently developing an in vivo coinfection model. The goal of this work is to identify genes and mechanisms that enable or disrupt S. aureus and P. aeruginosa coexistence for potential treatment targets for Cystic Fibrosis coinfections that often lead to some the worst complications of the lifelong genetic disease.