Protocols

Please consider sending your protocols to Rick Kahn .

(1)    Using CRISPR to KO genes in MEFs

This protocol describes the overall design and generation of MEFs knocked out for target genes. Please consult other protocols for details of each step in the process.

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(2)    Transfection of immortalized MEFs: Lipofectamine 2000 and PEI

This protocol walks you through our two different means of transfecting MEFs and uses for CRISPR, immunofluorescence, live cell imaging, or other procedures.

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(3)    Cloning MEFs by limited dilution

This is our procedure for generating clones after transfection and puromycin selection/enrichment in the CRISPR KO process. It can be used for cloning for any purpose.

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